为探索食用菌滑菇菌种在栽培茶树菇所剩下的废料在固体培养基和液体培养基的不同比例下对滑菇的细胞活性的影响，利用MTT(3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐)的方法探讨废料比对于滑菇菌丝活性的影响。通过不同比例的废料代替其原配方中的碳源和氮源，调节固体培养基和液体培养基的比例分别为0%、20%、40%、60%、80%、100%。用单因素的实验方法，测定在相同培养基的不同废料下对滑菇菌种菌丝活性的影响，实验结果表明当废料含量占配方的20%时，菌丝生物量能达到较高水平，能有助于提高滑菇菌种的产量。同时，该研究实现了菌种栽培废料的绿色循环利用，减少了因丢置造成的环境污染和杂菌虫害等危害。关键词：培养基；MTT;废料；滑菇；菌丝AbstractIn order to explore the influence of the waste materials left by the cultivationof Agrocybe chaxingu on the cell activity of pholiota nameko in differentproportion of solid medium and liquid medium,the method of MTT (3-(4,5-dimethylthiazol-2)-2,5-diphenyltetrazolium bromide)was used toexplore the influence of waste materials ratio on the mycelial activity ofpholiota nameko.The carbon and nitrogen sources in the original formula arereplaced by different proportions of waste.The proportion of solid medium andliquid medium was 0%,20%,40%,60%,80%and 100%,respectively.Thesingle factor experiment method was used to determine the effect of differentwaste materials on the mycelial activity of shiitake.The results showed thatwhen the waste materials accounted for 20%of the formula,the mycelialbiomass could reach a higher level,which could help to improve the yield ofpholiota nameko.At the same time,the research realized the green recycling ofthe culture waste,and reduced the environmental pollution and other hazardscaused by the waste disposal.Key words:culture medium;MTT;waste material;pholiota nameko hyphae目录1.1食用菌滑菇及其应用现状1.2食用菌废料的应用....12材料和方法..…22.1材料22.1.2仪器设备...2.1.3药品32.2.1菌种的活化以及扩种32.2.3灭菌2.2.4接种及培养2.2.5MTT的测定..2.2.7采样样品菌丝浓度的计算2.2.8固体培养基菌丝日均生长速度测量2.2.9固体菌丝每天生长量的测定与计算2.2.10数据处理773.2培养基不同废料比例对菌丝生物量的影响93.2.2固体培养基菌丝每天生长速度103.2.3固体培养基菌丝SDH活性...103.2.4固体培养基每天菌丝生长量11