维普论文检测系统/IP PAPER CHECK SYSTEM罗非鱼链球菌病的病原分离及耐药性检测论文三稿【原文对照报告-大学生版】报告编号：f98c5574429cdac2检测时间：2021-05-1814：42：38检测字符数：17531作者姓名：无所属单位：仲恺农业工程学院（教务处）全文总相似比复写率他引率自引率专业术语检测结论：60.63%48.32%12.31%0.0%0.0%其他指标：自写率：39.37%高频词：球菌，链球，链球菌，罗非鱼，无乳典型相似文章：无指标说明：复写率：相似或疑似重复内容占全文的比重他引率：引用他人的部分占全文的比重自引率：引用自己已发表部分占全文的比重自写率：原创内容占全文的比重典型相似性：相似或疑似重复内容占全文总相似比超过30%专业术语：公式定理、法律条文、行业用语等占全文的比重总相似片段期刊：104博硕：76综合：4相似片段：222外文：0自建库：8互联网：30检测范围：中文科技期刊论文全文数据库中文主要报纸全文数据库中国专利特色数据库博士/硕士学位论文全文数据库中国主要会议论文特色数据库港澳台文献资源外文特色文献数据全库维普优先出版论文全文数据库互联网数据资源/互联网文档资源高校自建资源库图书资源古籍文献资源个人自建资源库年鉴资源IPUB原创作品时间范围：1989-01-01至2021-05-18维普论文检测系统VIP PAPER CHECK SYSTEM原文对照颜色标注说明：■自写片段■复写片段（相似或疑似重复）口引用片段（引用）■专业术语（公式定理、法律条文、行业用语等）仲恺农业工程学院本科生毕业论文（设计）仲恺农业工程学院毕业论文姓名张杨婉院（系）动物科技学院专业班级水产171学号201721824131指导教师苏友禄职称/职务教授论文答辩日期仲恺农业工程学院教务处制Isolation of tilapia streptococcus strains and detection of drug resistanceZhangYangwanCollege of Animal Science and Technology Zhongkai University of Agriculture and EngineeringGuangzhou,ChinaSupervisor:Prof.Su Youlu学生承诺书“本人郑重承诺：所呈交的毕业论文（设计）是本人在导师的指导下独立进行研究所取得的成果。除了文中已用特别标志加以标记的引述内容之外，本论文（设计）不含有任何其他个人或集体已经发表或撰写的研究成果。对本文研究做出过重要贡献的个人或集体，均已在文中以明确的方式标明。若在毕业论文（设计）的各项检查、评比中被发现有抄袭、剽窃或其他的违规行为，本人愿按学校有关规定接受处理，并承担相应的法律责任”。学生（签名）：2021年3月19日摘要链球菌病严重影响着罗非鱼养殖业的持续健康发展，造成巨大的经济损失。本实验主要通过对采集的罗非鱼样品进行菌株分离、鉴定及耐药性检测，旨在为罗非鱼链球菌病的防控提供科学依据。研究内容如下：1.罗非鱼链球菌病的病原分离及鉴定对分离菌株进行16 SrRNA基因扩增，基因序列分析表明，所分离的菌株中有20株为无乳链球菌(Streptococcusagalactiae),8株为海豚链球菌(Streptococcus iniae)。同源性分析发现，有些无乳链球菌属于不同株型。此外，将分离的无乳链球菌、海豚链球菌进行革兰氏染色，结果显示，所有菌株呈紫色，球状，单个、短或长链状排列的球形菌。对所分离的菌株进行溶血性测试，所有菌株均无溶血性。2维普论文检测系统VIP PAPER CHECK SYSTEM2.罗非鱼链球菌病的耐药性检测利用药敏分析试剂板对28株无乳链球菌、海豚链球菌进行耐药性检测。结果显示，这28株链球菌对8种抗生素的耐药性各有差异。大部分无乳链球菌对盐酸多西环素、磺胺间甲氧嘧啶钠和磺胺甲噁唑十甲氧苄啶最敏感，对硫酸新霉素、氟甲喹呈中度敏感，只有少部分无乳链球菌对盐酸多西环素、磺胺间甲氧嘧啶钠、磺胺甲噁唑十甲氧苄啶、硫酸新霉素和氟甲喹不敏感：相反，海豚链球菌都对恩诺沙星、盐酸多西环素、磺胺间甲氧嘧啶钠和磺胺甲噁唑十甲氧苄啶最敏感；对硫酸新霉素、甲砜霉素、氟苯尼考和氟甲喹呈现中度敏感，这也说明了海豚链球菌暂不具有抗药性。综上所述，本研究在发病罗非鱼上分离到了无乳链球菌和海豚链球菌。这两种病原在形态和导致的临床症状上无明显差异。在耐药性检测方面，我们发现无乳链球菌、海豚链球菌都对盐酸多西环素、磺胺间甲氧嘧啶钠和磺胺甲愿唑十甲氧苄啶最敏感，而对其他抗生素的敏感性各有差异。关键词罗非鱼无乳链球菌海豚链球菌病原分离耐药性检测AbstractStreptococcal disease seriously affects the sustainable and healthy development of tilapiaaquaculture,causing huge economic losses.This experiment is mainly based on the isolation,identification and drug resistance detection of the collected tilapia samples,aiming to provide ascientific basis for the prevention and control of tilapia milk-free and dolphin streptococcusdisease.The main studies are as follows:1.The eogenescation and identification of erythra streptococcus disease.The gene amplification of 16SrRNA genes was carried out on the isolated strains,and genesequence analysis showed that 20 of the isolated strains were Streptococcus agalactiae and 8 werestreptoccus iniae.Homology analysis revealed that some Streptococcus agalactiae belong to differentstrains.In addition,the isolated agalactia,Streptococcus iniae were Gram-stained,and the resultsshowed that all the strains were purple,spherical,single,short or long chain spherical bacteriaarranged.The isolated strains were tested for hemolysis,and all the strains were non-hemolytic.2.Detection of drug resistance of tilapia streptococcus disease.28 strains of Streptococcus agalactiae and Streptococcus dolphinus were tested for drugresistance using the drug sensitivity analysis reagent board.The results showed that the 28 strainsof Streptococcus had different resistance to 8 antibiotics.Most Streptococcus agalactiae are mostsensitive to doxycycline hydrochloride,sulfamonomethoxine sodium and sulfamethoxazoletrimethoprim,and are moderately sensitive to neomycin sulfate and flumequine.Streptococcus lactisis insensitive to doxycycline hydrochloride,sulfamonomethoxine sodium,sulfamethoxazoletrimethoprim,neomycin sulfate and flumequine;on the contrary,Streptococcus dolphin is insensitiveto enrofloxacin,Doxycycline hydrochloride,sulfamonomethoxine sodium and sulfamethoxazoletrimethoprim are the most sensitive;it is moderately sensitive to neomycin sulfate,thiamphenicol,florfenicol and flumequine,which It also shows that Streptococcus dolphin has no drug resistancefor the time being.In summary,this study isolated Streptococcus agalactiae and Streptococcus iniae in diseasedtilapia.The two pathogens have no obvious difference in morphology and clinical symptoms.In termsof drug resistance testing,we found that Streptococcus agalactiae and Streptococcus inus are themost sensitive to doxycycline hydrochloride,sulfamonomethoxine sodium,and sulfamethoxazoletrimethoprim,but are sensitive to other antibiotics Sex is different.Key words Tilapia Streptococcus agalactiae Streptococcus dolphin Pathogen isolation Resistancetesting3目录罗非鱼链球菌病的病原分离及耐药性检测7前言73