上海医疗器械高等专料学校毕业设计葡萄球菌的快速检测方法研究摘要金黄色葡萄球菌(Staphylococcus aureus)在l884年首次被发现，Rosenbach在固体培养基上培养出葡萄球菌，并在同年记录了它能引起食物中毒。1930年，研究人员又明确指出金黄色葡萄球菌引起食物中毒与其产生的肠毒素有关，据估计，进食者摄入约100g的肠毒素A即可出现中毒症状。典型的金黄色葡萄球菌呈球型，直径为0.8μm左右，显微镜下排列成葡萄串状，无芽胞、鞭毛，大多数也无荚膜，属于革兰氏阳性细菌。目前，随着国际食品贸易不断发展，食品中致病微生物检测方法的研究也在不断向前发展，除了传统的培养方法之外，近年来，人们采用免疫学、生物化学和分子生物学等先进技术进行了食品中致病微生物快速检测方法的研究。对于金黄色葡萄球菌也不例外，传统的检测方法主要采用细菌分离培养及生化鉴别，免疫学方法（如：酶联免疫、免疫转印、乳胶凝集等方法)因其快速、操作简便，逐步应用于食品卫生检测。但新的分子生物学技术已显示出其在食品卫生检测方面的独特优势。本文主要针对这四种快速检测方法展开一系列地研究。关键词：食品；金黄色葡萄球菌；快速检测方法食品中金黄色葡萄球菌的快速检测方法研究Methods of the rapid detection of Staphylococcus aureusABSTRACTStaphylococcus aureus(Staphylococcus aureus)was first discovered in 1884,Rosenbachin the solid culture medium Staphylococcus,and in the same yearrecorded it can cause foodpoisoning.In 1930,the researchers pointed out thatdue to Staphylococcus aureus enterotoxinfood poisoning and its producing,according to estimates,food intake of about 100ngenterotoxin A can appearpoisoning symptoms.Typical S.aureus is ball shape,diameter ofabout 0.8 u m,microscope arranged thyrsoid,non spore forming,flagella,most noncapsule,belonging to the gram positive bacteria.At present,with the continuousdevelopment of international food trade,pathogenic microorganism detection methods infood is also in constant development,in addition,traditional training methods in recentyears,people adopt advanced technology in immunology,biochemistry and molecularbiology were studied for the rapid detection of pathogenic microorganism in food.There is noexception for Staphylococcus aureus,traditional detection methods of bacterial isolationculture and identification of the biochemical,immunological methods (such as:ELISA,Western blot,latex agglutination method etc.)because of its fast,simple operation,andgradually applied to the detection of food hygiene.But the new techniques of molecularbiology has shown its unique advantages in the aspects of food hygiene detection.This papermainly launches a series of studies on the four kinds of rapid detection methods.Key Words:food,Staphylococcus aureus,rapid detection method上海医疗器械高等专科学校毕业设计目录摘要第1章绪论1.1研究背景…1.2研究意义…1.3研究内容.…31.4研究方法第2章金黄色葡萄球菌简介…52.1金黄色葡萄球菌生物学特征.52.2金黄色葡萄球菌的致病性…2.3金黄色葡萄球菌的流行病学…2.4金黄色葡萄球菌的耐药性分析第3章食品中金黄色葡萄球菌常用的检测方法73.1传统的检测方法…3.2免疫学检测方法73.3以分子生物学为基础的快速检测方法73.4商业化快速检测方法第4章金黄色葡萄球菌四种检测方法的案例及分析4.1传统的检测方法案例分析…4.2免疫学检测方法案例分析4.3以分子生物学为基础的快速检测方法案例分析.94.3.】材料与方法.943.2结果与分析…134.4商业化快速检测方法案例分析.164.4.1试验材料与设备…164.4.2试验及分析方法第5章结论2021参考文献…