维普论文检测系统VIP PAPER CHECK SYSTEM2-(咪唑-2'-基)吡啶-铜(II)配合物的合成及抗肿瘤作用【原文对照报告-大学生版】报告编号：494d785645194288检测时间：2021-04-2719：33：55检测字符数：18772作者姓名：邝毅锋所属单位：维普论文检测全文总相似比复写率他引率自引率专业术语检测结论：33.18%33.18%0.0%0.0%0.0%其他指标：自写率：66.82%高频词：配合，细胞，作用，配体，实验典型相似文章：无指标说明：复写率：相似或疑似重复内容占全文的比重他引率：引用他人的部分占全文的比重自引率：引用自己已发表部分占全文的比重自写率：原创内容占全文的比重典型相似性：相似或疑似重复内容占全文总相似比超过30%专业术语：公式定理、法律条文、行业用语等占全文的比重总相似片段期刊：72博硕：120综合：1相似片段：217外文：0自建库：0互联网：24检测范围：中文科技期刊论文全文数据库中文主要报纸全文数据库中国专利特色数据库博士/硕士学位论文全文数据库中国主要会议论文特色数据库港澳台文献资源外文特色文献数据全库维普优先出版论文全文数据库互联网数据资源/互联网文档资源高校自建资源库图书资源古籍文献资源个人自建资源库年鉴资源IPUB原创作品时间范围：1989-01-01至2021-04-27维普论文检测系统VIP PAPER CHECK SYSTEM原文对照颜色标注说明：■自写片段■复写片段（相似或疑似重复）口引用片段（引用）■专业术语（公式定理、法律条文、行业用语等）本科毕业论文2-(咪唑-2'-基)吡啶-铜(I)配合物的合成及抗肿瘤作用邝毅锋201412060214指导教师乐学义教授学院名称材料与能源学院专业名称应用化学论文提交日期2021年5月3日论文答辩日期2021年5月日摘要吡啶、咪唑类化合物的氮原子具有强配位能力，可以与多种金属离子发生配位得到稳定性好、立体结构多样、具有特殊性能的配合物，同时它们也可作为桥联配体和螯合配体构建各种新颖结构的配合物。由于吡啶、咪唑类化合物与许多天然生物分子结构相似，表现出低毒性和极高的生物活性，作为抗癌药物具有极大的开发潜力。有证据表明，许多芳香氮杂环与金属离子配位后，生物活性明显强于游离配体，而且芳基金属配合物易于修饰，可通过结构微调来调控配合物的抗肿瘤活性，因此研究吡啶、咪唑类金属配合物有重要应用价值。本文设计合成了2-（咪唑-2'-基）吡啶-铜（①I)配合物，采用元素分析法确定其分子的元素组成，得到分子式为C16H16C12CN609。通过紫外可见光谱、摩尔电导率、红外光谱和X射线单晶衍射等方法对配合物进行了表征，最终2维普论文检测系统VIP PAPER CHECK SYSTEM确定了配合物的分子结构，为两分子IPY与Cu(II)螯合形成扭曲的平面四边形构型，电解质类型为1：2型。本文研究了配合物与DNA的作用机理，通过电子吸收光谱、B竞争实验、KI荧光淬灭实验、粘度法以及分子对接模拟等方法，证明了配合物与DNA之间存在较弱的插入作用，结合常数Kb值为3.865×104L·mo1-1,表明该配合物可能通过插入DNA的碱基对中来抑制DNA复制增殖，从而发挥抗肿瘤活性。同时，本文重点研究了配合物的体外细胞毒性，运用了MTT法测定了配体、配合物及顺铂对四种肿瘤细胞(Hela、SGC-7901、Hepg2、BEL-7402)的体外细胞毒性，并与对人正常肝细胞(L02)的作用进行比较，以顺铂为阳性对照，发现配合物对人正常肝细胞的毒性略低于顺铂，且在BEL-7402(人肝癌细胞)组实验中，配合物(IC50=17.19±0.60如o1·L-1)表现出比顺铂(IC50=28.62士0.72如o1·L-1)更好的抗肿瘤活性，有望成为该方向的抗肿瘤药物。关键词：铜(II)配合物2-（咪唑-2'-基）吡啶晶体结构细胞毒性DNASynthesis and Antitumor Activity of 2-(imidazole-2'-yl)pyridine Copper(II)ComplexKuang Yifeng(College of Science,South China Agricultural University,Guangzhou 510642,China)Abstract:Nitrogen atoms of pyridine and imidazole derivatives have strong coordination ability,which can coordinate to various metal ions to obtain complexes with good stability,various three-dimensional structures and special properties.The pyridine and imidazole derivatives can also beused as bridging and chelating ligands to construct diverse novel structures of complexes.Pyridineand imidazole derivatives are considered to have great potential as anticancer drugs because oftheir low toxicity and high biological activity,which is due to their similarities in structurewith many natural biomolecules.It is reported that the biological activity of many aromaticnitrogen heterocycles ligands coordinated to metal ions is significantly stronger than the freeligands.Moreover,the aromatic nitrogen heterocycles moiety of metal complexes is easily modified,which is able to regulate the antitumor activity of the complexes by fine-tuning the structure ofthe ligands.Therefore,there is considerable application value to study the metal complexes ofpyridines and imidazoles.In this paper,2-(imidazole-2'-y1)pyridine-copper(II)complex has been designed andsynthesized.The elemental composition of the complex has been determined by elemental analysis,andthen gave the molecular formula:C16H16C12CuN609.The UV-Vis spectroscopy,molar conductivityexperiment,infrared spectroscopy and X-ray single crystal diffraction were empolyed to characterizethe complex.The result of X-ray single crystal diffraction showed that the molecules of thecomplex,two IPY bidentate ligands were chelated with the center Cu(II)ion,forming a twisted planequadrilateral structure.According to the result of molar conductivity experiment,the electrolytetype was 1:2 type.The interaction mechanism between the complex and DNA was also investigated inthis paper.Through UV absorption spectroscopy,EB competition binding assay,KI fluorescencequenching experiment,viscosity measurement and molecular docking simulation,it was proved thatthere is a weak insertion between the complex and DNA,and the binding constant Kb value was 3.865X104 L.mol-1.These results indicated that the complex could inhibit DNA replication andproliferation by inserting into the base pairs of DNA,thus exerting the anticancer activity.Moreover,this paper focused on the in vitro cytotoxicity of the complex.The in vitro cytotoxicityof the ligand,complex and cisplatin against four kinds of tumor cells (HeLa,SGC-7901,Hepg2 andBel-7402)was determined by MTT assay,compared with the human normal liver cells (L02).The resultrevealed that the toxicity of the complex to human normal liver cells was slightly lower than thatof cisplatin.In the BEL-7402 experimental group,the complex (IC50 17.19 0.60 umol.L-1)displayed better antitumor activity than cisplatin (IC50 28.62+0.72 mol.L-1),which isexpected to be an anticancer drug in this direction.Key words:Copper(II)Complex 2-(imidazole-2'-yl)pyridine Crystal Structure Cytotoxicity DNA目录1引言13