本科生毕业论文论文题目：海洋真菌Penicillium sp.T2-l85与Aspergillus sp.T2-186共培养代谢产物研究姓名：余燕芝学号：202011110226专化学（师范）年2020级学化学与化工学院完成日期：2024年4月指导教师：黎婉珊（副教授）本科生毕业论文独创性声明本人声明所呈交的毕业论文是本人在导师指导下进行的研究工作及取得的研究成果，除了文中特别加以标注和致谢的地方外，本论文中没有抄袭他人研究成果和伪造数据等行为。与我一同工作的同志对本研究所做的任何贡献均已在论文中作了明确的说明并表示谢意。论文作者签名：日期：20年月日本科生毕业论文使用授权声明海南师范大学有权保留并向国家有关部门或机构送交毕业论文的复印件和磁盘，允许毕业论文被查阅和借阅。本人授权海南师范大学可以将本毕业论文的全部或部分内容编入有关数据库进行检索，可以采用影印、缩印或其他复印手段保存、汇编毕业论文。论文作者签名：日期：20年月日指导教师签名：日期：20年月日海洋真菌Penicillium sp.T2-185与Aspergillus sp.T2-186共培养代谢产物研究作者：余燕芝指导教师：黎婉珊副教授(海南师范大学化学与化工学院，海口，571158)摘要：海洋真菌生存在独特的海洋环境中，已经成为获得新颖天然产物理想来源。随着对微生物研究的深入和基因组测序研究表明，微生物的合次级代谢产物的基因簇在单独培养时绝大部分未被表达，因此，目前从微生物资源发现的代谢产物总数远低于其代谢能力。做生物共培养技术是激活其沉默基因簇的有效策略之一，因此共培养技术已成为挖掘微生物代谢潜能的重要方法之一，本论文对海洋来源的两株真菌Penicillium sp.T2-185与Aspergillus sp..T2-l86共培养的规模发酵产物的化学成分进行了研究，通过硅胶柱层析、凝胶柱层析和高效液相色谱等技术分离纯化得到三个单体化合物，并通过核磁共振波谱对其进行结构鉴定，确定化合物13分别是B-eudesmol,隐球内酯(cryptosphaerolide)和pestaloquinols A,关键词：共培养；海洋真菌；天然产物；结构鉴定Study on the metabolites of co-culture of marine fungiPenicillium sp.T2-185 and Aspergillus sp.T2-186Author:Yanzhi Yu Tutor:Associate Professor Wanshan Li(College of Chemistry and Chemical Engineering,Hainan Normal University,Haikou,571158)Abstract:Marine fungi survive in unique marine environments and have become an ideal source forobtaining novel natural products.With the deepening of microbial research and genome sequencingstudies,it has been shown that the gene clusters of microbial secondary metabolites are mostly notexpressed when cultured alone.Therefore,the total number of metabolites discovered from microbialresources is far lower than their metabolic capacity.Microbial co culture technology is one of theeffective strategies to activate silenced gene clusters,therefore co culture technology has become one ofthe important methods to explore the metabolic potential of microorganisms.In this paper,the chemicalcomponents of the large-scale fermentation products co cultured by two marine fungi Penicillium sp.T2-185 and Aspergillus sp.T2-186 were studied.Three compounds were isolated and purified by silicagel column chromatography,gel column chromatography and high performance liquid chromatography,and their structures were identified by nuclear magnetic resonance spectroscopy.Compounds 1-3 wereidentified as B-eudesmol,cryptosphaerolides and pestaloquinols A.Keywords:Co-culturing,Marine fungi ,Natural products,structure identification