诚信声明我声明，所呈交的毕业论文（设计）是本人在老师指导下进行的研究工作及取得的研究成果。据我查证，除了文中特别加以标注和致谢的地方外，论文(设计)中不包含其他人已经发表或撰写过的研究成果，也不包含为获得其他教育机构的学位或证书而使用过的材料。我承诺，论文（设计）中的所有内容均真实，可信。毕业论文（设计）作者（签名）：年月日恩诺沙星注射液在羊体内的药代动力学【摘要】目的：本试验目的是通过开展某公司生产的恩诺沙星注射液在羊体内的药代动力学研究，获得恩诺沙星注射液在羊体内的药代动力学特征，客观、科学地评价受试药物的质量，给临床合理用药提供理论和实践参考。方法：选用健康羊随机分成肌肉注射给药(A组)和静脉注射给药(B组)2组，公母各半，单次给药，剂量均为2.5 mg/kg.b.w.。在给药前及给药后一定间隔时间内分别采集血液样品，使用LC-MSMS测定羊血浆样品中恩诺沙星和环丙沙星的浓度并用药动学软件Winnonlin5.2的非房室模型来处理，计算出每只试验羊的主要药代动力学参数、平均值(x)及标准差(S.D.),同时以血药浓度平均值对时间作药-时曲线图。结果：静脉给药组恩诺沙星C0为6372.95±2421.83ng/mL,AUCo分别为5799.57±1481.31hng/mL;恩诺沙星后代谢物环丙沙星的Cmx为142.35±46.71ng/mL,Tm为1.00±0.42h。肌肉给药组恩诺沙星Cmx为1375.10±387.56ng/mL,Tmx为0.94±0.12h,生物利用度(F)为93.82%±18.07%：恩诺沙星后代谢物环丙沙星Cmx为109.50±44.76ng/mL,Tmx为2.50±0.93h。肌肉注射恩诺沙星后环丙沙星的生物利用度(F)为115.34%±26.66%。恩诺沙星注射液肌肉注射给药后，在羊体内吸收速度快，吸收程度高，体内分布广泛，生物利用度高。【关键词】：恩诺沙星注射液：药代动力学：环丙沙星Pharmacokinetics of enrofloxacin injection in sheep[Abstract]Objective:The purpose of this experiment is to obtain thepharmacokinetic characteristics of enrofloxacin injection in sheep by conductingpharmacokinetic study in sheep,to objectively and scientifically evaluate the qualityof the tested drugs,and to provide theoretical and practical references for rationalclinical use.Methods:Healthy sheep were randomly divided into two groups:intramuscular administration (group A)and intravenous administration (group B),with half of the males and half of the females,and a single dose of 2.5 mg/kg-b.w.was administered.Blood samples were collected before and at certain intervals afteradministration,and the concentrations of enrofloxacin and ciprofloxacin in sheepplasma samples were determined using LC-MS/MS and processed with the non-atrialmodel of the pharmacokinetic software Winnonlin 5.2.The main pharmacokineticparameters,mean (x and standard deviation (S.D.)were calculated for each testsheep,and the mean blood concentrations were also plotted against time for drug-timegraphs.Results:The Co of enrofloxacin was 6372.95 2421.83 ng/mL and theAUCO-t was 5799.57+1481.31 h-ng/mL in the intravenous administration group,respectively;the Cmax of the post-enrofloxacin metabolite ciprofloxacin was 142.35+46.71 ng/mL with a Tmax of 1.00+0.42 h.In the intramuscular administrationgroup,enrofloxacin Cmax was 1375.10+387.56 ng/mL with a Tmax of 0.94+0.12 hand a bioavailability (F)of 93.82%+18.07%;the post-enrofloxacin metaboliteciprofloxacin Cmax was 109.50 +44.76 ng/mL with a The bioavailability (F)ofciprofloxacin after intramuscular enrofloxacin injection was 115.34%+26.66%.Afterthe intramuscular administration of enrofloxacin injection,it was rapidly absorbed insheep with high degree of absorption,wide distribution in vivo and highbioavailability.[keywords]Enrofloxacin injection Pharmacokinetics Ciprofloxacin