维普论文检测系统VIP PAPER CHECK SYSTEM橘青霉次生代谢产物中苯并呋喃酮衍生物的提取分离和核磁鉴定【原文对照报告-大学生版】报告编号：cdf0e960cc874ed0检测时间：2021-05-2322：33：43检测字符数：15192作者姓名：陈蝶所属单位：全文总相似比复写率他引率自引率专业术语检测结论：28.86%24.69%4.17%0.0%0.0%其他指标：自写率：71.14%高频词：活性，化合，化合物，产物，代谢典型相似文章：无指标说明：复写率：相似或疑似重复内容占全文的比重他引率：引用他人的部分占全文的比重自引率：引用自己已发表部分占全文的比重自写率：原创内容占全文的比重典型相似性：相似或疑似重复内容占全文总相似比超过30%专业术语：公式定理、法律条文、行业用语等占全文的比重总相似片段期刊：22博硕：46综合：2相似片段：84外文：0自建库：0互联网：14检测范围：中文科技期刊论文全文数据库中文主要报纸全文数据库中国专利特色数据库博士/硕士学位论文全文数据库中国主要会议论文特色数据库港澳台文献资源外文特色文献数据全库维普优先出版论文全文数据库互联网数据资源/互联网文档资源高校自建资源库图书资源古籍文献资源个人自建资源库年鉴资源IPUB原创作品时间范围：1989-01-01至2021-05-23维普论文检测系统VIP PAPER CHECK SYSTEM原文对照颜色标注说明：■自写片段■复写片段（相似或疑似重复）口引用片段（引用）■专业术语（公式定理、法律条文、行业用语等）本科毕业论文题目：橘青霉次生代谢产物中苯并呋喃酮衍生物的提取分离和核磁鉴定学院：医学院专业：药学学号：201709157014学生姓名：陈蝶指导教师：刘娟杨曦亮日期：二O二一年五月摘要目的通过对内生真菌橘青霉次生代谢产物的提取分离、纯化和波谱解析的深入研究，确定其次生代谢产物的结构，以期发现新的活性。方法①菌株的发酵：从PDA斜面培养基挑取橘青霉菌株，接种到100m1PDB培养基中，恒温，摇床培养2天，按5%接种量转移到发酵培养基中进行大规模发酵：②次生代谢产物的提取：在得到发酵液后，加入等体积的乙酸乙酯萃取，经旋转蒸发仪蒸发浓缩后得棕色粗提取物：③分离：粗提取物经葡聚糖凝胶L止20，用甲醇洗脱，合并相同部分得到6个组份，组份3经硅胶柱层析洗脱分离又得到8个组份：④纯化：将得到的组份进RP-PLC,以乙腈-水(20%-100%)洗脱得单体化合物：⑤结构鉴定：通过解析1H-MR、13C-MR等谱图，与参考文献对照确定结构。结果通过柱层析分离及高效液相色谱纯化等方法，从橘青霉次生代谢产物中得到一白色结晶状化合物，利用核磁共振等方法确定该化合物为7-Hydroxy-5-me thoxy-4,6-dimethylisobenzofuran-l(3)-one。结论7-Hydroxy-5-methoxy-4,6-dimethylisobenzofuran-l(3H)-one为内生菌橘青霉次生代谢产物中的一种成分，在橘青霉中首次发现，其活性还需进行深入研究。关键词：橘青霉：次生代谢产物：提取分离：结构鉴定：波谱解析2维普论文检测系统VIP PAPER CHECK SYSTEMAbstractObjective To determine the structure of the secondary metabolites of endophytic fungusPenicillium citrus through in-depth study on the extraction,purification and spectral analysis ofthe secondary metabolites of Penicillium citrus,in order to find new natural active components.Methods Ofermentation of strain Penicillium citrus was selected from PDA slant culture medium,inoculated into 100 mlPDB medium,incubated in constant temperature shaker for 2 days,andtransferred to fermentation medium for large-scale fermentation according to 5%inoculum.2extraction of secondary metabolites:the same volume of ethyl acetate was added to thefermentation broth,and the crude brown extract was evaporated and concentrated by rotaryevaporator.3separation:the crude extract was eluted with methanol by dextran gel LH-20,and 6components were obtained by merging the same part,and 8 components were obtained by silica gelcolumn chromatography.4purification:the obtained components were eluted with acetonitrile-water(20%100%)to obtain monomer compounds.5structure identification:the structure wasdetermined byanalyzing IH-NMR and 13C-NMR spectra and comparing with the references.Results A white crystalline compound was obtained from the secondary metabolites of Penicilliumby column chromatography and high performance liquid chromatography.The compound was identified as7-hydroxy-5-methoxy-4-dimethylisobenzofuran-1 (3H)-one by NMR.Conclusion 7-hydroxy-5-methoxy-4-methoxy-6-dimethylisobenzofuran-1 (3H)-one is one of thesecondary metabolites of endophytic Penicillium citrus,which is the first in Penicillium citrus,and its activity needs to be further studied.Key words:Penicillium citrus;Secondary metabolites;Extraction and separation:Structureidentification;Spectrum analysis目录前言11实验材料及仪器41.1实验菌株41.2培养基41.3实验耗材41.4实验仪器42实验方法及步骤52.1橘青霉的发酵52.2次生代谢产物的提取52.3次生代谢产物的分离52.3.1硅胶柱分离52.3.1.1拌样、装柱及上样52.3.1.2梯度洗脱52.3.2凝胶柱分离62.4纯化62.5化合物的波谱解析与结构鉴定62.5.1化合物的NMR谱图解析62.5.1.113C-MR谱图解析62.5.1.2DEPT谱图解析92.5.1.31H-NR谱图解析102.6结构分析123结果讨论144结论与展望15参考文献163