维普论文检测系统/IP PAPER CHECK SYSTEM辣木籽异硫氰酸酯衍生物抑制四种癌细胞增殖的作用研究【原文对照报告-大学生版】报告编号：e187a1bbc35b421b检测时间：2021-04-2616：24：10检测字符数：14629作者姓名：姚忠莲所属单位：云南农业大学全文总相似比复写率他引率自引率专业术语检测结论：35.74%28.86%6.88%0.0%0.0%其他指标：自写率：64.26%高频词：细胞，形成，调亡，抑制，克隆典型相似文章：无指标说明：复写率：相似或疑似重复内容占全文的比重他引率：引用他人的部分占全文的比重自引率：引用自己已发表部分占全文的比重自写率：原创内容占全文的比重典型相似性：相似或疑似重复内容占全文总相似比超过30%专业术语：公式定理、法律条文、行业用语等占全文的比重总相似片段期刊：74博硕：58综合：1相似片段：168外文：0自建库：7互联网：28检测范围：中文科技期刊论文全文数据库中文主要报纸全文数据库中国专利特色数据库博士/硕士学位论文全文数据库中国主要会议论文特色数据库港澳台文献资源外文特色文献数据全库维普优先出版论文全文数据库互联网数据资源/互联网文档资源高校自建资源库图书资源古籍文献资源个人自建资源库年鉴资源IPUB原创作品时间范围：1989-01-01至2021-04-26维普论文检测系统VIP PAPER CHECK SYSTEM原文对照颜色标注说明：■自写片段■复写片段（相似或疑似重复）引用片段（引用）■专业术语（公式定理、法律条文、行业用语等）辣木籽异硫氰酸酯衍生物抑制四种癌细胞增殖的作用研究姚忠莲(云南农业大学食品科学技术学院，昆明650201)摘要【研究的目的】辣木是一种药食兼用的的热带多功能植物，异硫氰酸酯化合物是其主要活性成分是是之一，具有抗炎、抗癌、降血压等多种活性，但是由于辣木异硫氰酸酯(MITC)存在见光易分解，结构不稳定等问题，严重阻碍了该活性因子的开发与利用。因此，课题组前期对MIT℃进行化学结构修饰，并得到多种喹唑啉酮类衍生物。抗癌活性研究发现，MITC衍生物[6,8-二溴-2-硫代-3-(4-鼠李糖基苯甲基)-2,3-二氢喹唑啉-4-酮](MITC-21)具有较强的抗癌活性。本课题以MITC-21为实验材料，深入研究了其对四种癌细胞生长的抑制作用，以期为辣木籽资源的深度利用和开发奠定理论基础，也为癌症的防治提供科学依据。【方法】本课题采用MTT法研究经不同浓度的MITC-21(0、0.5、1、2、4、8和16μM)处理后四种癌细胞的存活率。采用克隆形成实验研究MITC-21(IC50值2.70μM)对四种癌细胞克隆形成的影响。采用流式细胞术检测经MITC-21(细胞IC50值对应浓度2.70μM)处理后的四种癌细胞(U251、A431、HCT-116和He1a)的凋亡变化情况。【结果】与对照组相比，MITC-21以剂量依赖性的方式显著抑制U251、A431、HCT-116和He1a细胞生长，其半抑制浓度(IC50)分别为2.70μM、8.03μM、8.60μM和6.34μM。克隆形成实验结果表明，MITC-21(IC50浓度)显著抑制四种细胞克隆形成，U251细胞克隆形成率从100%降低到18%(p<0.001),A431细胞克隆形成率从100%降低到11%(p<0.001),HCT-116细胞克隆形成率从100%降低到18%(p<0.001),Hela细胞克隆形成率从100%降低到8%(p<0.001)。流式细胞术的检测结果表明，MITC-21(IC50浓度)显著诱导四种细胞凋亡，U251细胞的凋亡率从15.83%增加到24.86%(p<0.001),A431细胞的凋亡率从14.82%增加到19.86%(p<0.001),HCT-116细胞的凋亡率从16.98%增加到29.68%(p<0.001),He1a细胞的凋亡率从8.93%增加到15.9%(p<0.001)。【结论】MITC-21对结肠癌HCT-116细胞、脑胶质瘤U251、皮肤鳞状细胞癌A431和宫颈癌Hela细胞均具有抑制增殖和诱导调亡的作用，其中MITC-21对U251细胞的抑制作用最强。关键词：辣木籽异硫氰酸酯(MITC);喹唑啉酮衍生物：宫颈癌Hela;结肠癌HCT-116;脑胶质瘤U251;皮肤鳞状细胞癌A431;细胞凋亡Moringa Oleifera Seed Isothiocyanate Derivatives Inhibit the Proliferation of Four Kinds ofCancer CellsYao Zhong Lian(College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China)ABSTRACT[OBJECTIVE]Moringa Oleifera is a tropical multifunctional plant for medicine and food.Isothiocyanate compound is one of its main active ingredients,which has various activities such as2维普论文检测系统VIP PAPER CHECK SYSTEManti-inflammatory,anti-cancer and lowering blood pressure,However,moringa isothiocyanate (MITC)has problems such as easy decomposition when exposed to light and unstable structure,whichseriously hinders the development and utilization of this active factor.Therefore,the researchgroup modified the chemical structure of MITC in the early stage and obtained a variety ofquinazolinone derivatives.Anti-cancer activity research found that,MITC derivative [6,8-dibromo-2-thio-3-(4-rhamnosylbenzyl)-2,3-dihydroquinazolin-4-one](MITC-21)has Strong anti-cancer activity.This subject uses MITC-21 as the experimental material,In-depth study of its inhibitory effect onthe growth of four kinds of cancer cells,In order to lay a theoretical foundation for the in-depthutilization and development of Moringa seed resources,It also provides scientific basis for cancerprevention and treatment.[METHOD]This subject uses the MTT method to study the survival rates offour cancer cells treated with different concentrations of MITC-21 (0,0.5,1,2,4,8 and 16 HM).The colony formation experiment was used to study the effect of MITC-21 (IC50 value 2.70 uM)on thecolony formation of four cancer cells.Flow cytometry was used to detect the changes in apoptosis offour cancer cells (U251,A431,HCT-116 and Hela)treated with MITC-21 (cell IC50 value correspondingto a concentration of 2.70 HM).[RESULTS]Compared with the control group,MITC-21 significantlyinhibited the growth of U251,A431,HCT-116 and Hela cells in a dose-dependent manner,The semi-inhibitory concentrations (IC50)were 2.70 uM,8.03 uM,8.60 uM and 6.34 uM,respectively.Theresults of the clone formation experiment showed that,MITC-21 (2.70 HM)significantly inhibitedthe formation of four kinds of cell clones,U251 cell clone formation rate decreased from 100%to18%(p<0.001),The clone formation rate of A431 cells decreased from 100%to 11%(p<0.001),Theclone formation rate of HCT-116 cells decreased from 100%to 18%(p<0.001),The clone formation rateof Hela cells decreased from 100%to 8%(p<0.001).The results of flow cytometry showed that,MITC-21 (2.70 uM)significantly increased the apoptosis of four kinds of cells,The apoptosis rate ofU251 cells increased from 15.83%to 24.86%(p<0.001),The apoptosis rate of A431 cells increasedfrom 14.82%to 19.86%(p<0.001),The clone formation rate of HCT-116 cells decreased from 100%to18%(p<0.001),The clone formation rate of Hela cells decreased from 100%to 8%(p<0.001).Theresults of flow cytometry showed that,MITC-21 (2.70 HM)significantly increased the apoptosis offour kinds of cells,The apoptosis rate of U251 cells increased from 15.83%to 24.86%(p<0.001),Theapoptosis rate of A431 cells increased from 14.82%to 19.86%(p<0.001),The apoptosis rate of HCT-116 cells increased from 16.98%to 29.68%(p<0.001),The apoptosis rate of Hela cells increased from8.93%to 15.9%(p<0.001).[CONCLUSION]MITC-21 can inhibit the proliferation and induce apoptosisof colon cancer HCT-116 cells,glioma U251,skin squamous cell carcinoma A431 and cervical cancerHela cells.In the MTT experiment,MITC-21 significantly inhibited the survival rate of U25 cells,and the IC50 at 48 h was 2.70 uM.In the clone formation experiment,MITC-21 (IC50 2.70 uM)hadthe most significant inhibitory efficiency on Hela cells,and the inhibitory rate reached 92%.Inflow cytometry,MITC-21 (IC50 2.70 uM)significantly increased the apoptosis rate of HCT-116 cells,which increased from 16.98%to 29.68%.Key words:Moringa seed isothiocyanate (MITC);quinazolinone derivatives;cervical cancer Hela;colon cancer HCT-116;glioma U251:skin squamous cell carcinoma A431;apoptosis辣木籽异硫氰酸酯衍生物抑制四种癌细胞增殖的作用研究1引言1.1辣木籽简介辣木是一种药食兼用的的热带植物，它不仅营养丰富，且药用价值极高[1]。我国已有部分地区大面积种植，国内外近年来对辣木研究很多，证实辣木的树皮、叶、果实都具有很强的生物活性[2】。有巨大的开发潜力。被科学界誉为“奇迹之树”[3]。辣木在我国的关注度日益增强。是这几年在欧州一些国家流行的保健食用植物，具有抗菌、抗氧化、抗炎、抗癌和降血压等活性[4幻。但国内现阶段多用于净化水质和作为绿色食品。国外近年来对它研究也很多，美国和英国的一些大学都有详细的研究报告。研究报告中显示辣木籽富含多种活性成分，对人体健康十分有3