诚信声明我声明，所呈交的毕业论文（设计）是本人在老师指导下进行的研究工作及取得的研究成果。据我查证，除了文中特别加以标注和致谢的地方外，论文（设计)中不包含其他人已经发表或撰写过的研究成果，也不包含为获得其他教育机构的学位或证书而使用过的材料。我承诺，论文（设计）中的所有内容均真实、可信。毕业论文（设计）作者（签名）：Poly(TMOS-co-MPTMS)杂化硅胶整体柱的制备研究【摘要】目的：固相萃取技术是一种样品前处理技术，发展至今已有不同种类的固相萃取技术。而固相萃取材料是固相萃取技术的研究热点，其中的有机-无机杂化材料兼具有机聚合物整体材料和硅胶整体材料的优势。溶胶-凝胶法是制备有机-无机杂化材料应用最广泛的方法，该法可以简单、高效、经济地制备出杂化材料。其中由TMOS和MPTMS所制备的杂化材料性能良好。本文旨在制备Poly(TMOS-co-MPTMS)杂化硅胶整体柱，并将其应用于亚甲蓝的吸附。方法：合成Poly(TMOS-co-MPTMS)杂化硅胶整体柱，通过考察pH值、缓冲盐种类和缓冲盐浓度对Poly(TMOS-co-MPTMS)杂化硅胶整体柱吸附亚甲蓝的影响，确定最优吸附条件，考察脱附效果。结果：成功制备出了Poy(TMOS-co-MPTMS)硅胶整体柱，确立了最优吸附条件。在pH为7.0的15mM的磷酸盐缓冲液的条件下对亚甲蓝吸附达82.1%。结论：制备的Poly(TMOS-co-MPTMS)硅胶整体柱在磷酸盐缓冲液中对亚甲蓝具有吸附能力，去除率为82.1%。可使用甲醇对吸附有亚甲蓝的Poly(TMOS-co-MPTMS)杂化硅胶整体柱进行脱附，脱附率为73.1%。【关键词】杂化硅胶整体柱：溶胶-凝胶法：亚甲蓝Preparation of Poly (TMOS-co-MPTMS)Monolithic HybridSilica Column[Abstract]Purpose:Solid-phase extraction technology is a pre-sampleprocessing technology,which has developed different types of solid-phase extractiontechnology so far.The solid-phase extraction material is a research hotspot ofsolid-phase extraction technology.Among them,the organic-inorganic hybridmaterials have the advantages of organic polymer materials and silica materials.Thesol-gel method is the most widely used method for preparing organic-inorganic hybridmaterials.This method can prepara hybrid materials,efficient,and economicallyprepared to produce hybrid materials.Among them,the hybrid materials prepared byTMOS and MPTMS have good performance.This article intends to prepare themonolithic hybrid silica column of poly (TMOS-co-MPTMS)and apply it for theadsorption of MB.Method:Poly (TMOS-co-MPTMS)monolithic silica column wereprepared for the adsorption of MB.By investigating the effects of pH value,buffersalt types and buffer salt concentration on adsorption capacity,the optimal adsorptionconditions were determined and the desorption effect was investigated.Result:Poly(TMOS-co-MPTMS)monolithic hybrid silica column were successfully prepared,andthe optimal adsorption conditions were established.The percentage of MB adsorptionis 82.1%in 15 mM phosphate buffer,pH 7.0.Conclusion:The prepared poly(TMOS-co-MPTMS)monolith can adsorb MB in phosphate buffer and the percentageof MB adsorption is 82.1%.MB was eluted from the poly (TMOS-co-MPTMS)monolithic hybrid silica column using methanol,with a desorption rate of 73.1%.[Keywords]Monolithic Hybird Silica ColumnSol-gel MethodMethyltltioninium Chlorid