诚信声明我声明，所呈交的毕业论文（设计）是本人在老师指导下进行的研究工作及取得的研究成果。据我查证，除了文中特别加以标注和致谢的地方外，论文（设计）中不包含其他人已经发表或撰写过的研究成果，也不包含为获得其他教育机构的学位或证书而使用过的材料。我承诺，论文（设计）中的所有内容均真实、可信。毕业论文（设计）作者（签名）：年月化妆品中脱水穿心莲内酯琥珀酸半酯的测定【摘要】目的：依据国家药监局批准《化妆品中脱水穿心莲内酯琥珀酸半酯的测定》(BJH202205)化妆品补充检验方法对化妆品中是否含有脱水穿心莲内酯琥珀酸半酯进行检测。方法：配制脱水穿心莲内酯琥珀酸半酯基质标准系列溶液，采用质谱检测器检测离子对峰面积定量，拟合标准曲线。进行空白试验，平行试验，回收试验，进行方法学的考察。将样品使用甲醇有机溶剂超声分散提取，使用高效液相色谱仪进行分离处理，并且用质谱检测器进行检测，根据保留时间和特征离子对的相对丰度比定性判断，根据定量离子对的峰面积定量计算，以标准曲线法计算得到最终的含量结果。结果：该法能有效检测化妆品中脱水穿心莲内酯琥珀酸半酯，检测限结果符合要求，样品精密度高，线性关系良好、重复性、稳定性良好。结论：检测样品均未检出脱水穿心莲内酯琥珀酸半酯，该测定方法适用于化妆品中脱水穿心莲内酯琥珀酸半酯的化验。【关键词】脱水穿心莲内酯琥珀酸半酯：高效液相色谱：三重四极杆质谱：标准曲线法Determination of dehydroandrographolide succinatein cosmetics[Abstract]Objective:According to the State Food and Drug Administrationapproved "Determination of dehydrated andrographolide half succinate in cosmetics"(BJH202205)cosmetic supplement test method to detect whether cosmetics containdehydrated andrographolide half succinate.Methods:The standard series solution ofdehydrated andrographolide half succinate matrix was prepared,and the quantifiedpeak area of ion pairs was detected by mass spectrometer,and the standard curve wasfitted.Conduct blank test,parallel test,recovery test,and methodologicalinvestigation.The samples were extracted with methanol organic solvent by ultrasonicdispersion,separated by high performance liquid chromatography,and detected bymass spectrometer.The retention time and relative abundance ratio of characteristicion pairs were qualitatively judged,the peak area of quantitative ion pairs wasquantitatively calculated,and the final content was calculated by standard curvemethod.Results:The method can effectively detect dehydrated andrographolide halfsuccinate in cosmetics,and the detection limit results meet the requirements.Thesample has high precision,good linear relationship,good repeatability and goodstability.Conclusion:No dehydrated andrographolide half succinate was detected inthe samples.This method is suitable for the determination of dehydratedandrographolide half succinate in cosmetics[Key words]High performance liquid chromatography Triple quadrupole massspectrometry Standard curveDehydroandrographolide succinate