诚信声明我声明，所呈交的毕业论文（设计）是本人在老师指导下进行的研究工作及取得的研究成果。据我查证，除了文中特别加以标注和致谢的地方外，论文(设计)中不包含其他人已经发表或撰写过的研究成果，也不包含为获得其他教育机构的学位或证书而使用过的材料。我承诺，论文（设计）中的所有内容均真实，可信。毕业论文（设计）作者（签名）：年月日金属铱配合物[Ir(bzq)2(DIPPA)]PF6合成及其抗肿瘤活性研究【摘要】目的：设计合成金属铱配合物[(bzq)2(DIPPA)PF6及研究其抗肿瘤活性。方法：以DPPA为配体，设计并合成了金属铱配合物[r(bzq)z(DIPPA)]PF6,采用HR-MS、HNMR,等方法对其进行表征，选取HCT116、LO2、B16、BEL-7402、A549、HepG2、Hela这七种细胞，用MTT法测定铱配合物对这7种细胞的毒性作用，通过细胞内吞和细胞周期阻滞实验，初步探索铱配合物对HCT116细胞的抗癌活性。结果：MTT体外毒性筛选实验表明铱配合物对所选的肿瘤细胞具有明显毒性，其中对HCT116细胞的毒性最强，因此将HCT116细胞作为后续实验的研究对象。细胞内吞实验表明铱配合物能够被HCT116细胞摄取，细胞周期实验表明铱配合物将HCT116细胞的周期阻滞在S期。结论：铱配合物[r(bzq)2(DIPPA)]PF6具有抑制HCT116细胞增殖的作用，并将HCT116细胞生长阻滞于S期。【关键词】铱配合物：细胞毒性；线粒体定位；抗癌活性Synthesis of Metal Iridium Complex [Ir(bzq)2(DIPPA)]PF6Antitumor Activity Study[Abstract]Objective:To design and synthesize the metal iridiumcomplex [Ir (bzq)(DIPPA)]PF6 and to study its antitumor activity.Methods:UsingDIPPA as a ligand,Designed and synthesized the metal iridium complex[Ir(bzq)2(DIPPA)]PF6,By using the HR-MS,!H NMR,And other methods tocharacter them.Subsequently,HCT116,LO2,B16,BEL-7402,A549,HepG2,Helawere selected,The toxic effects of iridium complexes on the above seven cells weredetermined by MTT assay,By the cell endocytosis and cell cycle arrest assay,Initially explore the anticancer activity of iridium complexes in HCT116 cells.Results:MTT in vitro toxicity screening experiment showed that iridium complexesshowed obvious toxicity to the selected tumor cells,which was the most toxic toHCT116 cells,so HCT116 cells were used as the following experiments.Cellendocytosis experiments showed that the iridium complex is efficiently up taken byHCT116 cells,and cell cycle experiments showed that iridium complexes arrest thecycle of HCT116 cells in S phase.Conclusion:Iridium complex [Ir(bzq)2(DIPPA)]PF6 suppresses HCT116 cell proliferation and blocks HCT116 cellgrowth in S phase.[Keywords]Iridium Complexes cytotoxic mitochondrial-localizedanticancer activity