诚信声明我声明，所呈交的毕业论文（设计）是本人在老师指导下进行的研究工作及取得的研究成果。据我查证，除了文中特别加以标注和致谢的地方外，论文（设计）中不包含其他人已经发表或撰写过的研究成果，也不包含为获得其他教育机构的学位或证书而使用过的材料。我承诺，论文（设计）中的所有内容均真实、可信。毕业论文（设计）作者（签名）：年月紫杉醇PLGA纳米粒的制备及体外释放研究【摘要】目的：以乳酸-羟基乙酸共聚物(PLGA)为载体，制备包载紫杉醇的PLGA纳米粒。方法：采用乳化-溶剂挥发法制备PLGA纳米粒，高效液相色谱法测定包封率及载药量。考察PLGA用量、二氯甲烷和乙醇的比值、PLGA型号及分子量和油相用量四个因素对样品粒径、包封率和载药量的影响。采用透析袋法进行体外释放度考察：高效液相色谱法测定体外释放度。结果：本课题所制得的纳米粒的平均包封率为(97.51±0.03)%、平均粒径为(195.57±1.23)nm、平均载药率为(0.98±0.01)%。结论：本文所用的实验方法制备而得的紫杉醇PLGA纳米粒粒径大小适宜且均匀，包封率和载药率良好，体外释放结果良好，可用于紫杉醇PLGA纳米粒的制备及体外释放研究。【关键词】紫杉醇；PLGA;HPLC;体外释放Preparation and in vitro release of paclitaxel PLGAnanoparticle[Abstract]Objective:Paclitaxel loaded PLGA nanoparticle were preparedwith PLGA as the carrier.Methods:PLGA nanoparticle were prepared by emulsionsolvent evaporation method.Examine the effects of four factors:PLGA dosage,ratio ofdichloromethane to ethanol,PLGA model and molecular weight,and oil phase dosage onsample particle size,encapsulation efficiency,and drug loading.Use dialysis bag methodfor in vitro release assessment:Determine in vitro release by high-performance liquidchromatography.Results:The average entrapment efficiency of the preparednanoparticle was(97.51 +0.03)%,the average particle size was (195.57 +1.23)nm,andthe average drug loading rate was (0.98 +0.01)%.Conclusion:The paclitaxel PLGAnanoparticles prepared by the experimental method in this paper have appropriate anduniform particle size,good encapsulation efficiency and drug loading rate,and good invitro release results,which can be used for the preparation and in vitro release ofpaclitaxel PLGA nanoparticle.Key words]Paclitaxel PLGA HPLC In vitro release