目录摘要关键词前言3第一章实验部分51.1实验试剂及仪器1.2溶液的配制51.3实验过程…1.3.1以牛血清蛋白为模板的金纳米簇的制备1.3.2疏脲(Tu)抑制BSA-AuNCs-Cu2+S2Og2体系荧光猝灭.61.3.3其他物质对抑制BSA-AuNCs-Cu2+-S2Og2-I体系荧光猝灭第二章结果与讨论62.1BSA-ANCs的表征2.2BSA-AuNCs间接检测硫脲>2.3实验条件的优化.…92.4BSA-AuNCs荧光探针的选择性第三章总结17参考文献18致谢20苏州大学本科生毕业论文基于荧光金纳米簇的高灵敏度疏脲检测摘要近年来随着纳米技术的发展，纳米材料作为一种新型荧光材料被广泛运用到分析领域山。目前已研究发现的荧光纳米材料主要包括以下几种：有机染料、聚合物点、碳量子点、半导体量子点、荧光金属纳米簇(FM NCs)等2-]。金纳米簇是一种尺寸小于3m的一种新型荧光材料，因其具有易合成、光稳定性高、低毒性等突出优势，被广泛用于各种目标物的检测。在本论文中，我们以牛血清蛋白为模板合成荧光金纳米簇。研究发现在向金纳米簇中加入碘离子、过硫酸盐以及铜离子时，其荧光发生明显的猝灭：如果同时加入硫脲，荧光发生恢复。通过荧光金纳米簇的恢复程度，可以实现对硫脲的灵敏检测。在最佳条件下，荧光的猝灭程度与硫脲的浓度呈一定的线性关系，其线性范围是0.24.0M,检测限可达0.03uM,有望实现实样检测。关键词：金纳米簇，荧光增强，过硫酸盐，碘离子，铜离子，硫脲苏州大学本科生毕业论文AbstractIn recent years,with the development of nanotechnology,nanomaterials have been widely used in the analysis field as a kind ofnovel fluorescent material.The fluorescent nanomaterials that have beenstudied so far include mainly organic dyes,polymer dots,carbon quantumdots,semiconductor quantum dots,fluorescent metal nanoclusters (FMNCs)and so on.Gold nanoclusters are a novel fluorescent material with asize of less than 3 nm.They are widely used for the detection of varioustargets due to their outstanding advantages such as easy synthesis,highlight stability,and low toxicity.In this paper,we synthesize fluorescent gold nanoclusters usingbovine serum albumin as a template.When iodide,persulphate,andcopper ions are added to gold nanoclusters,the fluorescence issignificantly quenched.However,when thiourea is added at the sametime,the fluorescence was recovered.Under an optimized condition,theextent of quenching was found linearly related to thiourea concentrationin the range of 0.2-4.0 uM,and thiourea as low as 0.03 uM could bedetected.This method is expect to implement real sample testing.Keywords:Gold nanoclusters;Fluorescence enhancement:Iodine;Copper ions:Thiourea