诚信声明我声明，所呈交的毕业论文（设计）是本人在老师指导下进行的研究工作及取得的研究成果。据我查证，除了文中特别加以标注和致谢的地方外，论文(设计)中不包含其他人已经发表或撰写过的研究成果，也不包含为获得其他教育机构的学位或证书而使用过的材料。我承诺，论文（设计）中的所有内容均真实、可信。毕业论文（设计）作者（签名）：年月日山竹壳中总氧杂蒽酮提取工艺优化及其对氧化损伤修复作用研究【摘要】目的：优化山竹壳中总氧杂蒽酮的提取工艺，并探究其对氧化损伤的修复作用。方法：采用单因素试验方法对山竹壳中的总氧杂蒽酮提取工艺进行优化，以总氧杂蒽酮含量为指标，确定最佳提取条件：戊唑醇建造氧化损伤模型，评价肝脏中谷胱甘肽的含量作为肝氧化损伤的修复指标：通过电化学分析法测定其脑组织中多巴胺的含量，并采用mage】软件对斑马鱼自发运动轨迹及运动参数进行分析，作为神经损伤的修复指标。结果：采用单因素优化提取条件，得到最佳提取条件为：提取时间为1.5h、料液比为1：60g/mL、提取温度为50℃、乙醇体积分数为80%：模型组斑马鱼肝组织的GSH含量相较空白组下降34.9%，不同剂量的给药组相较模型组分别上升12.1%、23.1%和49.8%。此外，相较于空白组，模型组斑马鱼脑组织中多巴胺含量提升81%，给药后，斑马鱼脑组织中多巴胺含量基本恢复至空白组水平。斑马鱼自发运动轨迹及运动参数恢复至正常水平。结论：总氧杂蒽酮可显著促进斑马鱼肝脏氧化损伤的修复，并对斑马鱼的神经氧化损伤也具有一定的修复作用。【关键词】山竹壳：氧杂蒽酮：提取工艺：氧化应激修复作用：神经毒性修复作用Optimization of extraction technology of total xanthonefrom mangosteen shell and its repair effect on oxidativedamageAbstract Objective:To optimize the extraction process of totalxanthone from mangosteen shell and explore its repairing effect on oxidative damage.Methods:The single-factor test method was used to optimize the extraction processof total xanthone in mangosteen shell,and the content of total xanthone was used asthe index to determine the optimal extraction conditions.Tebuconazole was used as anoxidative damage model in zebrafish,and the content of glutathione in the liver wasevaluated as an indicator of liver oxidative damage repair.At the same time,thedopamine content in the brain tissue was determined by electrochemical analysis,andthe spontaneous movement trajectory and movement parameters of zebrafish wereanalyzed by ImageJ software as indicators of neural damage repair.Results:Afteroptimizing the extraction conditions by single factor,the optimal extraction conditionswere:extraction time of 1.5h,liquid-to-material ratio of 1:60g/mL,extractiontemperature of 50C,and ethanol volume fraction of 80%.Compared with the blankgroup,the GSH content in the liver tissue of the model group zebrafish decreased by34.9%,and the different doses of the drug group increased by 12.1%,23.1%,and49.8%,respectively.In addition,compared with the blank group,the dopaminecontent in the brain tissue of the model group zebrafish increased by 8.1%.Afteradministration,the dopamine content in the brain tissue of zebrafish basically returnedto the level of the blank group.The spontaneous movement trajectory and movementparameters of zebrafish returned to normal levels.Conclusion:Total xanthone cansignificantly promote the repair of liver oxidative damage in zebrafish and also have acertain repairing effect on neural oxidative damage in zebrafish.[Key words]:Mangosteen shell xanthone Extraction technology Oxidative stressrepair Neurotoxic repair effect